Bioanalytics : Analytical Methods and Concepts in Biochemistry and Molecular Biology

Description
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids.

The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird’s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists.
The following techniques are covered in detail:\

Purification and determination of proteins
Measuring enzymatic activity
Microcalorimetry
Immunoassays, affinity chromatography and other immunological methods
Cross-linking, cleavage, and chemical modification of proteins
Light microscopy, electron microscopy and atomic force microscopy
Chromatographic and electrophoretic techniques
Protein sequence and composition analysis
Mass spectrometry methods
Measuring protein-protein interactions
Biosensors
NMR and EPR of biomolecules
Electron microscopy and X-ray structure analysis
Carbohydrate and lipid analysis
Analysis of posttranslational modifications
Isolation and determination of nucleic acids
DNA hybridization techniques
Polymerase chain reaction techniques
Protein sequence and composition analysis
DNA sequence and epigenetic modification analysis
Analysis of protein-nucleic acid interactions
Analysis of sequence data
Proteomics, metabolomics, peptidomics and toponomics
Chemical biology